Elucidation and Physicochemical Analysis of Bioactive Composition of Leaves and Root Methanol Extract of Robinia Pseudoacacia (Black Locust)

• Author(s): Patricia Ijeoma CHIME ; Evaristus Nnamno OWOH
• Paper ID: 1707136
• Page: 340-350
• Published Date: 15-02-2025
• Published In: Iconic Research And Engineering Journals
• Publisher: IRE Journals
• e-ISSN: 2456-8880
• Volume/Issue: Volume 8 Issue 8 February-2025

Abstract

The Elucidation and physicochemical analysis of bioactive composition of leaves and root methanol extract of Robinia pseudoacacia (black locust) is carried out successfully. All chemicals and reagents used in this study were of analytical grade. Mean Proximate Composition for both the leaves and the root gives (%)Protein14.88±0.09, 20.91±0.18; (%)Ash 2.71±0.02, 1.94±0.09; (%)Moisture 28.28±0.51, 19.81±0.13; (%)Fats22.13±0.05, 10.06±0.25; and (%)Fibre 0.28±0.04, ND. Mean Phytochemical Composition for both the leaves and the root respectively gives (%)Saponin 5.54±0.13, 0.11±0.16; (mg/g)Flavonoids 5.73±0.09, 1.87±0.02; (?g/g)Steroids 33.64±0.22, 27.98±0.35; (%)Alkaloid 39.39±0.86, 8.00±0.62; (%)Tannin 0.30±0.01, 0.22±0.002; and (mg/100g)Glycoside 65.11±1.39, 27.54±0.23. Acute toxicity test was carried out on both leaves and root extract of the plant. For both Stage 1 and Stage 2 of the test, none of the animals died and there were no signs of intoxication in all the mice. Chronic toxicity test was also done for both leaf and root extracts and the results showed no sign of intoxication in the tested mice. Functional groups composition of the Robinia pseudoacacia was also determined. Both the leaves and roots moderately contain alkene groups. The leaves have more phenolic and amino groups than that of the roots. Carboxylic acid is contained in both the leaves and the roots but it is more pronounced in the leaves. There is no alcoholic group in the leaves but it is present in the root at a very low concentration. Aldehyde group is present in the leaves but absent in the roots. This research revealed that the Robinia pseudoacacia (Black Locust) tree grown in the eastern part of Nigeria is not toxic.

Keywords

Robinia pseudoacacia, physicochemical, bioactive compound, Acute Toxicity, Chronic Toxicity

Citations

IRE Journals:
Patricia Ijeoma CHIME , Evaristus Nnamno OWOH "Elucidation and Physicochemical Analysis of Bioactive Composition of Leaves and Root Methanol Extract of Robinia Pseudoacacia (Black Locust)" Iconic Research And Engineering Journals Volume 8 Issue 8 2025 Page 340-350

IEEE:
Patricia Ijeoma CHIME , Evaristus Nnamno OWOH "Elucidation and Physicochemical Analysis of Bioactive Composition of Leaves and Root Methanol Extract of Robinia Pseudoacacia (Black Locust)" Iconic Research And Engineering Journals, 8(8)