Analytical Method Development and Validation for Simultaneous Estimation of 5-methyl-N-{[2-(morpholin-4-yl)quinolin-3-yl]methyl}-1,3-thiazol-2-amine in Bulk Form
  • Author(s): Yogita Shinde ; Kalpana Patankar-Jain
  • Paper ID: 1703742
  • Page: 106-111
  • Published Date: 15-09-2022
  • Published In: Iconic Research And Engineering Journals
  • Publisher: IRE Journals
  • e-ISSN: 2456-8880
  • Volume/Issue: Volume 6 Issue 3 September-2022
Abstract

A reverse phase high performance liquid chromatographic technique for validating 5-methyl-N-[2-(morpholin-4-yl)quinolin-3-yl]methyl-1,3-thiazol-2-amine (MMQMTA) in its original form has been developed. A HYPERSIL (250 x 4.6 mm, 5 m) ID column was used for the chromatography, and the mobile phase was a 60:40 mixture of methanol and ammonium acetate with a flow rate of 1.0 ml/min. The detection wavelength was 235 nm. MMQMTA had a retention time of 6.812 min. The approach yields linearity responses at concentrations of 4–24 mg/ml of MMQMTA. MMQMTA has a detection limit of 0.021 g/ml and an area of 2.61. Area 6.812 and 0.05 g/ml were reported as the MMQMTA limits of quantification, and the MMQMTA recovery rate was determined to be 99.98%. The technique is helpful for ensuring and monitoring the quality of pharmaceutical and bulk formulations.

Keywords

5-methyl-N-{[2-(morpholin-4-yl)quinolin-3-yl]methyl}-1,3-thiazol-2-amine, MMQMTA, RP-HPLC, validation.

Citations

IRE Journals:
Yogita Shinde , Kalpana Patankar-Jain "Analytical Method Development and Validation for Simultaneous Estimation of 5-methyl-N-{[2-(morpholin-4-yl)quinolin-3-yl]methyl}-1,3-thiazol-2-amine in Bulk Form" Iconic Research And Engineering Journals Volume 6 Issue 3 2022 Page 106-111

IEEE:
Yogita Shinde , Kalpana Patankar-Jain "Analytical Method Development and Validation for Simultaneous Estimation of 5-methyl-N-{[2-(morpholin-4-yl)quinolin-3-yl]methyl}-1,3-thiazol-2-amine in Bulk Form" Iconic Research And Engineering Journals, 6(3)