Malaria is one of the most common infectious diseases and public health problems around the globe. Malaria parasite is transmitted by Anopheles mosquitoes classified under Family Culicidae of Order Diptera and. The Anopheles complex has been particularly incriminated as one of the important malaria vectors in Sri Lanka and other part of the island. The present review provides basic aspects to differentiate morphologically identified sibling species of the Anopheles complex by different DNA based molecular techniques such as random amplified polymorphic DNA, restriction fragment length polymorphisms. DNA barcoding and Polymerase chain reaction. Moreover, it is particularly useful when using small amount of starting materials from particular specimen or from immature stages. Molecular technique provides a powerful tool for effective examination on the population dynamics of mosquitoes. It enables more detailed understanding on the relationships between the vectorial capacity, genetic makeup and geographical origin for a particular species of Anopheles, more detailed and precise taxonomy, as well as evolutionary studies.
Anopheles, Malaria, Mosquitoes, Molecular techniques, DNA
Kothai Kaveevendan "Discrimination Of The Anopheles Sp. Using Different Molecular Techniques" Iconic Research And Engineering Journals Volume 3 Issue 8 2020 Page 46-56
Kothai Kaveevendan "Discrimination Of The Anopheles Sp. Using Different Molecular Techniques" Iconic Research And Engineering Journals, 3(8)