The production of the lignin degrading enzymes namely Laccase, Manganese Peroxidase (MnP) and Lignin Peroxidase (LiP) of selected white-rot fungus were examined in this study. Three fungal cultures were studied, two of which belong to Ganoderma sp. & one belongs to Schizophyllum sp.. After screening the cultures for the presence of the enzymes Laccase by Plate Assay techniques, quantitative analysis of all the three enzymes was done by Spectrophotometric method. Production was studied using liquid media. The effect of pH & different carbon sources namely Sugarcane bagasse, Rice bran and Wheat bran was examined on the production of these enzymes in liquid media. Effect of 2, 5-xylidine on Laccase production was also assessed. As it is these enzymes that help in decolourization / degradation of dyes, the crude enzyme solution was also investigated for its ability to decolorize selected dyes. All the cultures showed diverse enzyme production of all the three enzymes in different media & pH. All the selected dyes were decolorized by the crude enzyme extract in varying degrees. This study demonstrated that media manipulation can change the enzyme production in the fungal cultures. By optimizing the media condition we can maximize the desired enzyme production. The study also shows that rather than using fungi, is possible to directly use crude enzymes for decolourization of dyes & probably treat industrial effluents.
Laccase, Manganese Peroxidase (MnP), Lignin Peroxidase (LiP), Reactive dyes, Effluent, Mycelia, Ganoderma sp., Schizophyllum sp.
Vijaya Nadagouda , Mrugesh D Shukla , Jatin Parikh "Study Of Production Of Lignin Degrading Enzymes By Fungal Cultures And Their Ability To Decolorize Selected Dyes" Iconic Research And Engineering Journals Volume 1 Issue 2 2017 Page 14-26
Vijaya Nadagouda , Mrugesh D Shukla , Jatin Parikh "Study Of Production Of Lignin Degrading Enzymes By Fungal Cultures And Their Ability To Decolorize Selected Dyes" Iconic Research And Engineering Journals, 1(2)