This study analysed the effects of conductor transposition on the 132kv Alaoji-Port Harcourt transmission lines. This study was motivated on the grounds that the inductance and capacitance of conductors will not be equal if they are not transposed at regular intervals. When parallel conductors, such as power lines, are run in parallel with transmission lines, excessive voltages can be induced in the lines. As a result, acoustic shock or noise may occur. This unfavourable phenomenon is considerably reduced by transposition. Also, the Spacing between conductors in a transmission line is not same in practice which also causes limitation in the transmission of energy. However, conductors are not usually transposed in transmission lines in practice. The switching stations and substations are where the transposition takes place. In Nigeria, One of the major challenges affecting the transmission line system is the line losses and continual vandalism of the 132KV transmission lines throughout the country's various areas the article gives an examination of a 132kV transmission line with and without transposition using Matlab and the EMTP (Electromagnetic Transients Program), particularly Bergeron's basic constant parameter model. Findings from this analysis shows that the unbalance obtained in this case without transposition is 1.39%. The results with transposition differ very little, but those without transposition have a deviation of 1.39%; which exceeds the maximum allowable unbalance value. The findings support the continuation of research in this direction, and they can also be used to the challenge of determining the allowed imbalance level in longer lines. The study suggests that in order to obtain a favorable outcome when balancing power flows via phases and circuits of multi-conductor lines, additional techniques for conductor transposition should be investigated in order to reach a better degree of line-wide equilibrium.